Review



peripheral blood mononuclear cell isolation kit  (Beijing Solarbio Science)


Bioz Verified Symbol Beijing Solarbio Science is a verified supplier
Bioz Manufacturer Symbol Beijing Solarbio Science manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 93
      Buy from Supplier

    Structured Review

    Beijing Solarbio Science peripheral blood mononuclear cell isolation kit
    Vaccine-induced humoral and cellular immune responses in piglets. (A) Serum PEDV-specific IgG antibody titers determined by 10-fold serial dilution ELISA. The dotted line represents a serum ratio of 0.8, above which samples were considered positive. (B) <t>PBMC</t> proliferation-associated metabolic activity measured by CCK-8 assay. PBMCs were isolated at day 14 post-boost, cultured in RPMI-1640 medium, and stimulated with PEDV-S protein (10 µg/mL) for 72 h. (C) IFN-γ levels in PBMC culture supernatants quantified by ELISA following PEDV-S protein stimulation. (D) IL-4 levels in PBMC culture supernatants quantified by ELISA following PEDV-S protein stimulation Data are shown as mean ± SD (n = 6 per group). Statistical significance: ***p < 0.001.
    Peripheral Blood Mononuclear Cell Isolation Kit, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 93/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pbmc+isolation+solution+kit/Porcine+Peripheral+Blood+Mononuclear+Cell+Isolation+Solution+Kit/pmc13071001-73-14-20
    Average 93 stars, based on 12 article reviews
    peripheral blood mononuclear cell isolation kit - by Bioz Stars, 2026-08
    93/100 stars

    Images

    1) Product Images from "A needle-free intradermal PEDV S protein vaccine elicits protective lactogenic immunity in piglets"

    Article Title: A needle-free intradermal PEDV S protein vaccine elicits protective lactogenic immunity in piglets

    Journal: Frontiers in Cellular and Infection Microbiology

    doi: 10.3389/fcimb.2026.1720440

    Vaccine-induced humoral and cellular immune responses in piglets. (A) Serum PEDV-specific IgG antibody titers determined by 10-fold serial dilution ELISA. The dotted line represents a serum ratio of 0.8, above which samples were considered positive. (B) PBMC proliferation-associated metabolic activity measured by CCK-8 assay. PBMCs were isolated at day 14 post-boost, cultured in RPMI-1640 medium, and stimulated with PEDV-S protein (10 µg/mL) for 72 h. (C) IFN-γ levels in PBMC culture supernatants quantified by ELISA following PEDV-S protein stimulation. (D) IL-4 levels in PBMC culture supernatants quantified by ELISA following PEDV-S protein stimulation Data are shown as mean ± SD (n = 6 per group). Statistical significance: ***p < 0.001.
    Figure Legend Snippet: Vaccine-induced humoral and cellular immune responses in piglets. (A) Serum PEDV-specific IgG antibody titers determined by 10-fold serial dilution ELISA. The dotted line represents a serum ratio of 0.8, above which samples were considered positive. (B) PBMC proliferation-associated metabolic activity measured by CCK-8 assay. PBMCs were isolated at day 14 post-boost, cultured in RPMI-1640 medium, and stimulated with PEDV-S protein (10 µg/mL) for 72 h. (C) IFN-γ levels in PBMC culture supernatants quantified by ELISA following PEDV-S protein stimulation. (D) IL-4 levels in PBMC culture supernatants quantified by ELISA following PEDV-S protein stimulation Data are shown as mean ± SD (n = 6 per group). Statistical significance: ***p < 0.001.

    Techniques Used: Serial Dilution, Enzyme-linked Immunosorbent Assay, Activity Assay, CCK-8 Assay, Isolation, Cell Culture



    Similar Products

    peripheral blood mononuclear cell isolation kit  (Beijing Solarbio Science)
    93
    Beijing Solarbio Science peripheral blood mononuclear cell isolation kit
    Vaccine-induced humoral and cellular immune responses in piglets. (A) Serum PEDV-specific IgG antibody titers determined by 10-fold serial dilution ELISA. The dotted line represents a serum ratio of 0.8, above which samples were considered positive. (B) <t>PBMC</t> proliferation-associated metabolic activity measured by CCK-8 assay. PBMCs were isolated at day 14 post-boost, cultured in RPMI-1640 medium, and stimulated with PEDV-S protein (10 µg/mL) for 72 h. (C) IFN-γ levels in PBMC culture supernatants quantified by ELISA following PEDV-S protein stimulation. (D) IL-4 levels in PBMC culture supernatants quantified by ELISA following PEDV-S protein stimulation Data are shown as mean ± SD (n = 6 per group). Statistical significance: ***p < 0.001.
    Peripheral Blood Mononuclear Cell Isolation Kit, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pbmc+isolation+solution+kit/Porcine+Peripheral+Blood+Mononuclear+Cell+Isolation+Solution+Kit/pmc13071001-73-14-20
    Average 93 stars, based on 1 article reviews
    peripheral blood mononuclear cell isolation kit - by Bioz Stars, 2026-08
    93/100 stars
    		  Buy from Supplier
    blood mononuclear cell isolation kit  (Beijing Solarbio Science)
    94
    Beijing Solarbio Science blood mononuclear cell isolation kit
    Vaccine-induced humoral and cellular immune responses in piglets. (A) Serum PEDV-specific IgG antibody titers determined by 10-fold serial dilution ELISA. The dotted line represents a serum ratio of 0.8, above which samples were considered positive. (B) <t>PBMC</t> proliferation-associated metabolic activity measured by CCK-8 assay. PBMCs were isolated at day 14 post-boost, cultured in RPMI-1640 medium, and stimulated with PEDV-S protein (10 µg/mL) for 72 h. (C) IFN-γ levels in PBMC culture supernatants quantified by ELISA following PEDV-S protein stimulation. (D) IL-4 levels in PBMC culture supernatants quantified by ELISA following PEDV-S protein stimulation Data are shown as mean ± SD (n = 6 per group). Statistical significance: ***p < 0.001.
    Blood Mononuclear Cell Isolation Kit, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pbmc+isolation+solution+kit/Equine+Peripheral+Blood+Mononuclear+Cell+Isolation+Solution+Kit/pm41821422-318-10-15
    Average 94 stars, based on 1 article reviews
    blood mononuclear cell isolation kit - by Bioz Stars, 2026-08
    94/100 stars
    		  Buy from Supplier
    human peripheral blood mononuclear cell isolation kit  (Beijing Solarbio Science)
    96
    Beijing Solarbio Science human peripheral blood mononuclear cell isolation kit
    Vaccine-induced humoral and cellular immune responses in piglets. (A) Serum PEDV-specific IgG antibody titers determined by 10-fold serial dilution ELISA. The dotted line represents a serum ratio of 0.8, above which samples were considered positive. (B) <t>PBMC</t> proliferation-associated metabolic activity measured by CCK-8 assay. PBMCs were isolated at day 14 post-boost, cultured in RPMI-1640 medium, and stimulated with PEDV-S protein (10 µg/mL) for 72 h. (C) IFN-γ levels in PBMC culture supernatants quantified by ELISA following PEDV-S protein stimulation. (D) IL-4 levels in PBMC culture supernatants quantified by ELISA following PEDV-S protein stimulation Data are shown as mean ± SD (n = 6 per group). Statistical significance: ***p < 0.001.
    Human Peripheral Blood Mononuclear Cell Isolation Kit, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pbmc+isolation+solution+kit/Human+Peripheral+Blood+Mononuclear+Cell+Isolation+Solution/pm41699973-38-18-26
    Average 96 stars, based on 1 article reviews
    human peripheral blood mononuclear cell isolation kit - by Bioz Stars, 2026-08
    96/100 stars
    		  Buy from Supplier
    mouse peripheral blood mononuclear cell separation kit  (Beijing Solarbio Science)
    95
    Beijing Solarbio Science mouse peripheral blood mononuclear cell separation kit
    Vaccine-induced humoral and cellular immune responses in piglets. (A) Serum PEDV-specific IgG antibody titers determined by 10-fold serial dilution ELISA. The dotted line represents a serum ratio of 0.8, above which samples were considered positive. (B) <t>PBMC</t> proliferation-associated metabolic activity measured by CCK-8 assay. PBMCs were isolated at day 14 post-boost, cultured in RPMI-1640 medium, and stimulated with PEDV-S protein (10 µg/mL) for 72 h. (C) IFN-γ levels in PBMC culture supernatants quantified by ELISA following PEDV-S protein stimulation. (D) IL-4 levels in PBMC culture supernatants quantified by ELISA following PEDV-S protein stimulation Data are shown as mean ± SD (n = 6 per group). Statistical significance: ***p < 0.001.
    Mouse Peripheral Blood Mononuclear Cell Separation Kit, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pbmc+isolation+solution+kit/Mouse+Peripheral+Blood+Mononuclear+Cell+Isolation+Solution+Kit/pm41570786-82-0-7
    Average 95 stars, based on 1 article reviews
    mouse peripheral blood mononuclear cell separation kit - by Bioz Stars, 2026-08
    95/100 stars
    		  Buy from Supplier
    peripheral blood mononuclear cell pbmc isolation solution kit  (Beijing Solarbio Science)
    95
    Beijing Solarbio Science peripheral blood mononuclear cell pbmc isolation solution kit
    Vaccine-induced humoral and cellular immune responses in piglets. (A) Serum PEDV-specific IgG antibody titers determined by 10-fold serial dilution ELISA. The dotted line represents a serum ratio of 0.8, above which samples were considered positive. (B) <t>PBMC</t> proliferation-associated metabolic activity measured by CCK-8 assay. PBMCs were isolated at day 14 post-boost, cultured in RPMI-1640 medium, and stimulated with PEDV-S protein (10 µg/mL) for 72 h. (C) IFN-γ levels in PBMC culture supernatants quantified by ELISA following PEDV-S protein stimulation. (D) IL-4 levels in PBMC culture supernatants quantified by ELISA following PEDV-S protein stimulation Data are shown as mean ± SD (n = 6 per group). Statistical significance: ***p < 0.001.
    Peripheral Blood Mononuclear Cell Pbmc Isolation Solution Kit, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pbmc+isolation+solution+kit/Camel+Peripheral+Blood+Mononuclear+Cell+Isolation+Solution+Kit/10__1016_slash_j__cej__2026__173750-90-0-11
    Average 95 stars, based on 1 article reviews
    peripheral blood mononuclear cell pbmc isolation solution kit - by Bioz Stars, 2026-08
    95/100 stars
    		  Buy from Supplier
    mouse peripheral blood mononuclear cells pbmcs  (Beijing Solarbio Science)
    95
    Beijing Solarbio Science mouse peripheral blood mononuclear cells pbmcs
    Vaccine-induced humoral and cellular immune responses in piglets. (A) Serum PEDV-specific IgG antibody titers determined by 10-fold serial dilution ELISA. The dotted line represents a serum ratio of 0.8, above which samples were considered positive. (B) <t>PBMC</t> proliferation-associated metabolic activity measured by CCK-8 assay. PBMCs were isolated at day 14 post-boost, cultured in RPMI-1640 medium, and stimulated with PEDV-S protein (10 µg/mL) for 72 h. (C) IFN-γ levels in PBMC culture supernatants quantified by ELISA following PEDV-S protein stimulation. (D) IL-4 levels in PBMC culture supernatants quantified by ELISA following PEDV-S protein stimulation Data are shown as mean ± SD (n = 6 per group). Statistical significance: ***p < 0.001.
    Mouse Peripheral Blood Mononuclear Cells Pbmcs, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pbmc+isolation+solution+kit/Mouse+Peripheral+Blood+Mononuclear+Cell+Isolation+Solution+Kit/pmc12891123-93-4-17
    Average 95 stars, based on 1 article reviews
    mouse peripheral blood mononuclear cells pbmcs - by Bioz Stars, 2026-08
    95/100 stars
    		  Buy from Supplier
    mouse peripheral blood mononuclear cell isolation solution kit  (Beijing Solarbio Science)
    95
    Beijing Solarbio Science mouse peripheral blood mononuclear cell isolation solution kit
    Vaccine-induced humoral and cellular immune responses in piglets. (A) Serum PEDV-specific IgG antibody titers determined by 10-fold serial dilution ELISA. The dotted line represents a serum ratio of 0.8, above which samples were considered positive. (B) <t>PBMC</t> proliferation-associated metabolic activity measured by CCK-8 assay. PBMCs were isolated at day 14 post-boost, cultured in RPMI-1640 medium, and stimulated with PEDV-S protein (10 µg/mL) for 72 h. (C) IFN-γ levels in PBMC culture supernatants quantified by ELISA following PEDV-S protein stimulation. (D) IL-4 levels in PBMC culture supernatants quantified by ELISA following PEDV-S protein stimulation Data are shown as mean ± SD (n = 6 per group). Statistical significance: ***p < 0.001.
    Mouse Peripheral Blood Mononuclear Cell Isolation Solution Kit, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pbmc+isolation+solution+kit/Mouse+Peripheral+Blood+Mononuclear+Cell+Isolation+Solution+Kit/pm41520540-230-16-24
    Average 95 stars, based on 1 article reviews
    mouse peripheral blood mononuclear cell isolation solution kit - by Bioz Stars, 2026-08
    95/100 stars
    		  Buy from Supplier
    bovine peripheral blood mononuclear cells pbmc  (Beijing Solarbio Science)
    93
    Beijing Solarbio Science bovine peripheral blood mononuclear cells pbmc
    Vaccine-induced humoral and cellular immune responses in piglets. (A) Serum PEDV-specific IgG antibody titers determined by 10-fold serial dilution ELISA. The dotted line represents a serum ratio of 0.8, above which samples were considered positive. (B) <t>PBMC</t> proliferation-associated metabolic activity measured by CCK-8 assay. PBMCs were isolated at day 14 post-boost, cultured in RPMI-1640 medium, and stimulated with PEDV-S protein (10 µg/mL) for 72 h. (C) IFN-γ levels in PBMC culture supernatants quantified by ELISA following PEDV-S protein stimulation. (D) IL-4 levels in PBMC culture supernatants quantified by ELISA following PEDV-S protein stimulation Data are shown as mean ± SD (n = 6 per group). Statistical significance: ***p < 0.001.
    Bovine Peripheral Blood Mononuclear Cells Pbmc, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pbmc+isolation+solution+kit/Bovine+Peripheral+Blood+Mononuclear+Cell+Isolation+Solution+Kit/pm41478530-86-5-14
    Average 93 stars, based on 1 article reviews
    bovine peripheral blood mononuclear cells pbmc - by Bioz Stars, 2026-08
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    Vaccine-induced humoral and cellular immune responses in piglets. (A) Serum PEDV-specific IgG antibody titers determined by 10-fold serial dilution ELISA. The dotted line represents a serum ratio of 0.8, above which samples were considered positive. (B) PBMC proliferation-associated metabolic activity measured by CCK-8 assay. PBMCs were isolated at day 14 post-boost, cultured in RPMI-1640 medium, and stimulated with PEDV-S protein (10 µg/mL) for 72 h. (C) IFN-γ levels in PBMC culture supernatants quantified by ELISA following PEDV-S protein stimulation. (D) IL-4 levels in PBMC culture supernatants quantified by ELISA following PEDV-S protein stimulation Data are shown as mean ± SD (n = 6 per group). Statistical significance: ***p < 0.001.

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: A needle-free intradermal PEDV S protein vaccine elicits protective lactogenic immunity in piglets

    doi: 10.3389/fcimb.2026.1720440

    Figure Lengend Snippet: Vaccine-induced humoral and cellular immune responses in piglets. (A) Serum PEDV-specific IgG antibody titers determined by 10-fold serial dilution ELISA. The dotted line represents a serum ratio of 0.8, above which samples were considered positive. (B) PBMC proliferation-associated metabolic activity measured by CCK-8 assay. PBMCs were isolated at day 14 post-boost, cultured in RPMI-1640 medium, and stimulated with PEDV-S protein (10 µg/mL) for 72 h. (C) IFN-γ levels in PBMC culture supernatants quantified by ELISA following PEDV-S protein stimulation. (D) IL-4 levels in PBMC culture supernatants quantified by ELISA following PEDV-S protein stimulation Data are shown as mean ± SD (n = 6 per group). Statistical significance: ***p < 0.001.

    Article Snippet: At day 14 post-boost, peripheral blood mononuclear cells (PBMCs) were isolated using a porcine peripheral blood mononuclear cell isolation kit (Solarbio, China) according to the manufacturer’s instructions.

    Techniques: Serial Dilution, Enzyme-linked Immunosorbent Assay, Activity Assay, CCK-8 Assay, Isolation, Cell Culture